Whole-body/organ Imaging with Single-cell Resolution: Toward Organism-level Systems Biology


Hiroki Ueda, M.D., Ph.D.

Date and Location

Monday July 29, 2019 12:30pm
NRI (Bio 2), Room 4164


Hiroki Ueda, M.D., Ph.D., University of Tokyo, Graduate School of Medicine

"Whole-body/organ Imaging with Single-cell Resolution: Toward Organism-level Systems Biology"

Hosted by: The seminar is funded by a CNSI Challenge-Program Development Grant (Departments of Psychological & Brain Sciences, Statistics & Applied Probability, Materials).
Contact: janusonis@ucsb.edu, detering@ucsb.edu, cbates@ucsb.edu

State-of-the-art tissue clearing methods provide unprecedented, high-quality optical access to very large biological specimens from individual organs to entire intact animals. When combined with light-sheet microscopy and image informatics, currently available tissue-clearing methods, including a hydrophilic tissue clearing method CUBIC, allows us to perform whole-brain and whole-body imaging with sub-cellular resolution. In addition, delipidation of biological specimens by tissue-clearing methods enables whole-organ staining with specific antibodies, which can be applied even to human biology and pathology. When combined with tissue clearing, these labelling methods allow us to systematically extract structural and functional information of complex mammalian organs. In this talk, I discuss how tissue-clearing and its related technologies have been successfully integrated to create new biological insights and provide a perspective for future opportunities in biology and medicine.

References: https://www.ncbi.nlm.nih.gov/pubmed/30381424 and https://www.ncbi.nlm.nih.gov/pubmed/29507408